AVB100 is an E. coli K12 strain with the biotin ligase gene stably integrated into the chromosome.

AVB100, an E. coli K12 strain [MC1061 araD139 delta (ara-leu)7696 delta(lac)l74 galU galK hsdR2(rK-mK+) mcrB1 rpsL(StrR)], is a birA gene stably integrated into the chromosome. Overexpression of the BirA protein is accomplished by induction with L-arabinose. The stably integrated birA gene does not require antibiotics to be maintained, and use of AVB100 with IPTG-inducible vectors such as Avidity's pAC or pAN. AviTag vectors allows independent control over the expressed gene of interest and the BirA levels. Avidity, LLC provide glycerol stocks (AVB100) or electrocompetent cells (EVB100). Purchase of AVB100 includes a license for use only at site of purchase and may not be distributed to other sites. Learn more about this strain.